LIQUEFACTION AND SACCHARIFACTION PROCESSES

摘要

The present invention relates to a process for liquefying starch-containing material, comprising treating the starch-containing material with a bacterial alpha-amylase at a temperature between 70-90°C for 10-120 minutes and a pullulanase at a temperature in the range from 40-60°C for between 20 and 90 minutes. The invention also relates to a saccharification process for saccharifying liquefied starch-containing material, comprising saccharifying a liquefied starch-containing material in the presence of a carbohydrate-source generating enzyme and a pullulanase. Finally, the invention also relates to a process of producing a fermentation product, such as ethanol, comprising a liquefaction step and/or saccharification step carried out in accordance with the present invention.