СПОСОБ КОЛИЧЕСТВЕННОЙ ОЦЕНКИ СТИМУЛЯЦИИ КОЛЛАГЕНОГЕНЕЗА БИОПЛАСТИЧЕСКИМИ МАТЕРИАЛАМИ

摘要

FIELD: medicine. ^ SUBSTANCE: one should place bioplastic material in culture liquid with a culture of dermal fibroblasts to cultivate fibroblasts together with bioplastic material for 4 d, then it is necessary to sample culture liquid and detect the concentration of protein-bound hydroxyproline. One concludes upon the degree of collagenogenesis stimulation with tested bioplastic material by greater or lesser degree of increased concentration of protein-bound hydroxyproline in sampled culture liquid against the control. As a control sample it is useful to apply culture liquid in which one could observe the tested bioplastic material for 4 d but no fibroblasts. The innovation is simple and enables to quantitatively evaluate the stimulation of collagenogenesis with bioplastic materials without applying any laboratory animals. ^ EFFECT: higher accuracy of evaluation.