QUANTIFYING MRNA OF PROTEINS OF INTEREST USING A REAL-TIME RT-PCR REACTION

摘要

The invention concerns the field of quantification of RNA's in a biological sample, in particular quantification of messenger RNA's coding for cytokines. The invention enables the level of messenger RNA's of human cytokines present in a biological sample to be absolutely quantified, through a real-time RT-PCR technique based on the use of highly specific primers, the detection of amplifications by incorporating a fluorescent intercalator, and the absolute quantification by using internal (constitutive genes) and external reference RNA standard range) norms. The invention concerns the simultaneous quantification of different RNA's, in the same experimental conditions.