发明名称 |
Modified sarcosine oxidase, process for producing the same and reagent composition using the same |
摘要 |
A protein having been modified by addition, deletion, insertion or substitution of at least one amino acid in an amino acid sequence constituting a protein having a sarcosine oxidase activity and still having the sarcosine oxidase activity, characterized by having an improved stability in the state of a liquid compared with the unmodified one and/or having a lowered action on L-proline compared with the unmodified one. A sarcosine oxidase having at least one of the following characteristics, i.e., an action on L-proline being 0.7% or less based on sarcosine and a Km value to L-proline being 150 mM or more, when measured at 37° C. and pH 8.0; a process for producing sarcosine oxidase having an excellent substrate specificity which comprises culturing a microorganism capable of producing sarcosine oxidase and collecting the sarcosine oxidase from the culture medium; and a reagent for measuring creatinine which contains the sarcosine oxidase.
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申请公布号 |
US2006051832(A1) |
申请公布日期 |
2006.03.09 |
申请号 |
US20050534583 |
申请日期 |
2005.05.11 |
申请人 |
TOYO BOSEKI KABUSHIKI KAISHA |
发明人 |
KISHIMOTO TAKAHIDE;SOGABE ATSUSHI;OKA MASANORI |
分类号 |
C12Q1/26;C07H21/04;C12N9/06;C12N15/53;C12P21/06;G01N33/70 |
主分类号 |
C12Q1/26 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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