发明名称 |
Multi-site mutagenesis |
摘要 |
The present invention provides compositions and improved methods for multi-site directed mutagenesis and DNA shuffling. The present compositions and methods provide increased mutation frequency and increased number of transformants which allow one to sequence only a few clones in order to identify the correct mutants and to obtain the desired mutant by screening large number of transformants in a short time. Moreover, the inclusion of FEN-1, PEF and optimized buffer and cycling conditions provided in the present invention should also facilitate random mutagenized library construction and the mutagenesis of large or difficult templates.
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申请公布号 |
US2006051748(A1) |
申请公布日期 |
2006.03.09 |
申请号 |
US20020198449 |
申请日期 |
2002.07.18 |
申请人 |
HOGREFE HOLLY H;CLINE JANICE M |
发明人 |
HOGREFE HOLLY H.;CLINE JANICE M. |
分类号 |
C12N15/09;C12Q1/68;C07H21/04;C12N9/12;C12N9/22;C12N15/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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