IMPROVED STRAINS OF E. COLI FOR PLASMID DNA PRODUCTION

摘要

A general method and strains of bacteria are described, by means of which it is possible to dramatically purify plasmid DNA with respect to genomic DNA, and RNA. In one preferred embodiment, lysis and nuclease removal of host nucleic acids is an integral component of the fermentation/harvest process, and as such, achieves increased yield and purity with simplified downstream purification and reduced waste streams, thus reducing production costs.