发明名称 |
METHOD FOR INDUSTRIALLY PRODUCING A JAPANESE ENCEPHALITIS VACCINE, AND RESULTING VACCINE |
摘要 |
A method is disclosed for industrially producing a Japanese encephalitis vaccine, wherein (a) cells from a cell line are cultured, (b) the resulting cell culture is inoculated with a Japanese encephalitis virus in the presence of a viral growth medium, (c) the virus is propagated and multiplied on the cells, (d) the viral growth medium is recovered in the form of a suspension of viruses produced by the cells, (e) the virus suspension is purified in at least one ion exchange chromatography step and a gel permeation step, and (f) the virus suspension is formulated and converted into a pharmaceutical form to preserve it until the moment of use. A Japanese encephalitis vaccine characterised in that it comprises a Japanese encephalitis virus produced by culturing cells from a cell line, and in that the cellular DNA content is less than 100 pg/dose, is also disclosed. |
申请公布号 |
WO9704803(A1) |
申请公布日期 |
1997.02.13 |
申请号 |
WO1996FR01195 |
申请日期 |
1996.07.29 |
申请人 |
PASTEUR MERIEUX SERUMS & VACCINS;FANGET, BERNARD;FRANCON, ALAIN;HEIMENDINGER, PIERRE |
发明人 |
FANGET, BERNARD;FRANCON, ALAIN;HEIMENDINGER, PIERRE |
分类号 |
A61K35/74;A61K39/12;C12N7/02;C12N7/06 |
主分类号 |
A61K35/74 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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