| 摘要 |
<p>Oligonucleotides are synthesized on the basis of amino acid sequences of the N-terminus of subunit I, and the N-terminus of subunit II of cytochrome bd type quinol oxidase of Brevibacterium flavum. PCR is performed by using the oligonucleotides as primers, and chromosome DNA of B. flavum as template, and a gene encoding cytochrome bd type quinol oxidase of B. flavum is obtained from a chromosome DNA library of Brevibacterium lactofermentum using the above obtained amplification fragment as a probe.</p> |
