Genetic regions associated with scrotal or inguinal hernia

摘要

Use of a first nucleic acid (I) to determine the predisposition to inherit the phenotype 'Hernia inguinalis/scrotalis' in a mammal is new. (I) has a length of at least 8 nucleotides and is identical or mainly identical to a second nucleic acid (II) which occurs in specified regions of chromosomes 12, 6, 3, 7 and 15 of Sus scrofa. Use of a first nucleic acid (I) to determine the predisposition to inherit the phenotype 'Hernia inguinalis/scrotalis' in a mammal is new. (I) has a length of at least 8 nucleotides and is identical or mainly identical to a second nucleic acid (II) which occurs: (a) on chromosome 12 of Sus scrofa in the region between 19.3 cM and 85.05 cM; (b) on chromosome 6 of Sus scrofa in the region between 71.4 cM and 96.1 cM or in the 0 cM region; (c) on chromosome 3 of Sus scrofa in the region between 12.4 cM and 19 cM, or 98.2 cM and 102,2 cM; (d) on chromosome 7 of Sus scrofa in the region between 58.5 cM and 59.7 cM; (e) on chromosome 15 of Sus scrofa in the region between 13.8 cM and 25.7 cM; or (f) on chromosomal region of another mammal, which is orthologous with regions as in (a)-(e) above. (I) is localized in the above regions or is 5 megabases upstream and/or downstream from them. Independent claims are also included for the following: (1) use of a combination of at least two or more of the above nucleic acids to determine the predisposition to inherit the phenotype 'Hernia inguinalis/scrotalis'; (2) an in vitro procedure for determining the predisposition to inherit the phenotype 'Hernia inguinalis/scrotalis'; (3) a kit comprising in one or more containers: (a') a primer pair for amplification of (II), where a primer binds at the + strand and a further primer binds at the - strand of (II); (b') a hybridization probe having a length of at least 8 nucleotides and which binds to (II); or (c') a specific antibody, antibody fragment, antibody derivative or an aptamer which binds to (II).