发明名称 |
NOVEL VECTOR |
摘要 |
<p>In order to provide a vector and a method for introducing one copy of a desired gene to a predetermined position of a host DNA at a high frequency without any bad influences upon the host cell and a vector and a method for deleting or inverting a host DNA at a predetermined position without a crossing step and without any bad influences upon the host cell. A vector which has an introduction cassette inserted between two site-specific recombinase recognition sequences and a site-specific recombinase gene which recognizes these site-specific recombinase recognition sequences, wherein the recombinase gene is positioned outside the introduction cassette, is used, and this is introduced into a host cell having a DNA in which one or two site-specific recombinase recognition sequences are present. A recombinant of interest can be efficiently obtained by positioning a lethal induction gene or a morphological abnormality induction gene outside the introduction cassette, together with the site-specific recombinase gene.</p> |
申请公布号 |
EP1630233(A1) |
申请公布日期 |
2006.03.01 |
申请号 |
EP20040745724 |
申请日期 |
2004.06.03 |
申请人 |
NIPPON PAPER INDUSTRIES CO., LTD. |
发明人 |
NANTO, KAZUYA;WATANABE, KEIKO;EBINUMA, HIROYASU |
分类号 |
C12N15/82;(IPC1-7):C12N15/82 |
主分类号 |
C12N15/82 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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