IMMUNOGENIC POLYPEPTIDE INDUCING PROTECTIVE IMMUNE RESPONSE AGAINST BACILLUS ANTHRACIS (VARIANTS), METHOD FOR ITS PREPARING, NUCLEIC ACID ENCODING THEREOF, EXPRESSION VECTOR (VARIANTS), METHOD AND VACCINE FOR PROPHYLAXIS OF INFECTION CAUSED BY BACILLUS ANTHRACIS

摘要

FIELD: biotechnology, molecular biology, microbiology, genetic engineering. ^ SUBSTANCE: invention relates to a method for preparing an immunogenic polypeptide inducing immune response that represents the protective response against infection with Bacillus anthracis. Proposed immunogenic polypeptide comprises from one to three domains of the full-scale Protective Antigen (PA) from B. anthracis or their variants and at least one of indicated domains represents domain 1 or domain 4 from PA or its variant. These variants of immunogenic polypeptide and full-scale PA are produced as result of expression in E. coli. Also, invention proposes a vector for expression in bacterial cells that comprises nucleic acid encoding abovementioned immunogenic polypeptide. Also, invention the developed method for prophylaxis of infection caused by B. anthracis based on administration of sufficient amount of immunogenic polypeptide. Also, invention proposes a vaccine for prophylaxis of infection caused by B. anthracis that comprises the effective amount of immunogenic polypeptide and a suitable carrier. Invention provides preparing the effective agent used for prophylaxis of infection caused by B. anthracis. ^ EFFECT: improved preparing method and valuable properties of polypeptide and vaccine. ^ 22 cl, 5 dwg, 3 tbl, 6 ex