METHOD OF ASSAYING a1,4-N-ACETYLGLUCOSAMINE TRANSFERASE (a4GnT) mRNA

摘要

It is intended to provide a method of assaying a1,4-N-acetylglucosamine transferase (a4GnT) mRNA contained in a sample characterized by comprising: (1) the step of using a first primer, which is homologous with at least a part in the downstream from the 5'-end of a specific base sequence of the above RNA, and a second primer, which is complementary to at least a part in the upstream from the 3'-end of the specific base sequence as described above, and forming a double-stranded DNA having a promoter sequence and the above specific base sequence in the downstream of the promoter sequence, wherein at least one of the first and second primers has a promoter sequence at the 5'-end; (2) the step of using the double-stranded DNA as a template and forming an RNA transcriptional product; (3) the step of successively using the RNA transcriptional product as a template for DNA synthesis and thus amplifying the RNA transcriptional product; and (4) the step of quantifying the above-described RNA transcriptional product.