WASHING METHOD OF SUPERCRITICAL FLUID CHROMATOGRAPHY SEPARATOR

摘要

PROBLEM TO BE SOLVED: To provide a washing method having a high washing effect and high washing convenience as compared with a conventional washing method in a washing method of a supercritical fluid chromatography separator.

SOLUTION: In the supercritical fluid chromatography separator having a separation part for separating a target substance from a sample using a supercritical fluid and a mobile phase containing a solvent and an extraction part for taking out the target substance from the mobile phase containing the target substance separated in the separation part, a back pressure valve is also provided on the downstream side of the extraction part in addition to a separating back pressure valve for adjusting the pressure of the mobile phase in the separation part. The internal pressures of the separation part and the extraction part are adjusted by the adjustment of pressure due to the back pressure valve and the mobile phase formed in the separation part is supplied to the extraction part to wash the extraction part.

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