METHODS AND KITS FOR ASSAYING MYELOPEROXIDASE BY MEANS OF ENZYMATIC GLYCOLATE-GLYOXYLATE CYCLING REACTIONS

摘要

The present invention provides a method for assaying myeloperoxidase activity. In the assay, a sample containing myeloperoxidase or suspected of containing myeloperoxidase is contacted with substrate including serine, hydrogen peroxide, and a halide. If myeloperoxidase is present in the sample, serine is converted into glycolaldehyde, which is further converted into glycolate by a glycoaldehyde converting enzyme (e.g. glycolaldehyde dehydrogenase) (1.2.1.21). The method then utilizes a cycling reaction system between glycolate and glyoxylate to generate a detectable signal that corresponds to the myeloperoxidase activity. Kits for assaying myeloperoxidases based on the same principle are also provided.