| 摘要 |
Use of an activated solid phase and an anchoring part which is attached to a peptide for solid phase peptide synthesis, wherein the anchoring part is coordinatively and reversibly attached to the activated solid phase. Furthermore provided is a process for competitively detachment of said anchoring part, for purification and refolding of said peptides. Provided are peptides with an anchoring part for coordinative and reversible attachment of said peptides to an activated solid phase. In preferred embodiments, the linkage between peptide(s) and solid phase consists of a metal chelate complex. The immobilized peptide is subjected to synthetic steps for sequence elongation (i. e. is a growing peptide). The preferred metal ion for chelate complexes is Cu(2+), and the preferred ligand on resin is 1,10-phenantroline. |
