Recombinant rabies viruses in which the arginine residue of the glycoprotein (G) at amino acid position 333 is exchanged, renders these viruses nonpathogenic for immunocompetent mammals regardless of the route of infection. Some of these recombinant rabies viruses after several serial virus passages in newborn mice can become pathogenic for adult mice. The reversion to the pathogenic phenotype is associated with a thymidine to adenosine mutation (TôA) at position 639 of the G gene, which results in an asparagine to lysine exchange at position 194 of G. The codon at position 637-639 was changed by site directed mutagenesis to replace asparagine at position 194 by an amino acid that minimized the possibility for an AsnôLys exchange at amino acid position 194 of G and prevents reversion to a pathogenic form of the virus.
申请公布号
WO2006017276(A2)
申请公布日期
2006.02.16
申请号
WO2005US24671
申请日期
2005.07.12
申请人
THOMAS JEFFERSON UNIVERSITY;DIETZSCHOLD, BERNHARD;FABER, MARIE LUISE;FABER, MILOSZ;PAK, KOON YAN;MATTIS, JEFFREY, A.
发明人
DIETZSCHOLD, BERNHARD;FABER, MARIE LUISE;FABER, MILOSZ;PAK, KOON YAN;MATTIS, JEFFREY, A.