The present invention relates to the cystic fibrosis gene and the field of genetic screening. More specifically, the described embodiments concern nucleic acids and proteins that encode novel mutations in the cystic fibrosis gene and methods to screen for the presence or absence of mutations or polymorphism's in the cystic fibrosis gene. Approaches to screen for the presence or absence of mutations that are correlated with cystic fibrosis and approaches to design primers that generate extension products that facilitate the resolution of multiple extension products in a single lane of a gel or in a single run on a column are also provided.
