MICROSCOPE OBSERVATION METHOD, MICROSCOPE, DIFFERENTIATION INTERFERENCE MICROSCOPE, PHASE DIFFERENCE MICROSCOPE, INTERFERENCE MICROSCOPE, IMAGE PROCESSING METHOD, AND IMAGE PROCESSING DEVICE

摘要

It is possible to obtain a special image such as a super-resolution image without specializing the configuration of a microscope. A microscope observation method includes: a measurement step for changing the illumination angle of an object (1) and measuring the complex amplitude distribution of each light wave generated separately on the image formation plane (15) by each light flux emitted from the object (1) at the respective illumination angle values; a calculation step for calculating the complex amplitude distribution of the virtual light wave generated on the image formation plane when the image formation optical system (14) is replaced by a virtual image formation optical system (L') having a greater numerical aperture according to the data on the complex amplitude distribution of each light wave; and an image creation step for creating image data on the virtual image formed on the image formation plane by the virtual image formation optical system (L') according to the complex amplitude distribution of the virtual light wave.