| 摘要 |
<p>The invention relates to bi-functionalised metallocenes of general formula (I) where Me=a transition metal, preferably chosen from Fe, Ru and Os, Y and Z, when identical are selected from -(CH<SUB>2</SUB>)<SUB>n</SUB>-O-, (CH<SUB>2</SUB>)-O-[(CH<SUB>2</SUB>)<SUB>2</SUB>-O]<SUB>P</SUB>- and -(CH<SUB>2</SUB>)<SUB>q</SUB>-CONH-(CH<SUB>2</SUB>)<SUB>r</SUB>-O-, or Y=-(CH<SUB>2</SUB>)<SUB>S</SUB>-NH- and Z=-(CH<SUB>2</SUB>)<SUB>t</SUB>-COO-, <SUB>n</SUB>=a whole number from 3 to 6 inclusive, p=a whole number from 1 to 4 inclusive, q=a whole number from 0 to 2 inclusive, r=a whole number from 0 to 2 inclusive, s=a whole number from 2 to 5 inclusive, t=a whole number from 3 to 6 inclusive, R and R'=H atoms or are protective groups used in oligonucleotide and peptide synthesis, where at least one of R or R' is protective group used in oligonucleotide and peptide synthesis and R and R' are as defined below: (i) when Z and Y are selected from (CH<SUB>2</SUB>)<SUB>n</SUB>-O-, -(CH<SUB>2</SUB>)-O-[(CH<SUB>2</SUB>)<SUB>2</SUB>-O]<SUB>p</SUB>- and -(CH<SUB>2</SUB>)<SUB>q</SUB>-CONH-(CH<SUB>2</SUB>)<SUB>r</SUB>-O-, then R and R' are protective groups used in oligonucleotide synthesis and R is a group which can leave a free OH group after deprotection, preferably a photolabile group such as monomethroxythoxytrityl, dimethoxytrityl, t-butyldimethylsilyl, acetyl or trifluroacetyl, and R' is a phosphorylated group which can react with a free OH, preferably a phosphodiester, phosphoramidite or H-phosphonate and (ii) when Y=-(CH<SUB>2</SUB>)<SUB>s</SUB>-NH- and Z=-(CH<SUB>2</SUB>)<SUB>t</SUB>-COO-, then R is a protective group used in the synthesis of peptides and is an amino-protecting group, preferably 9-fluorenyloxycarbonyl, t-butoxycarbonyl or benzyloxycarbonyl and R'=H. The above is applied in marking.</p> |
| 申请人 |
BIOMERIEUX |
发明人 |
CHAIX-BAUVAIS, CAROLE;MOUSTROU, CORINNE;NAVARRO, AUDE-EMMANUELLE;BRISSET, HUGUES;GARNIER, FRANCIS;MANDRAND, BERNARD;SPINELLI, NICOLAS |
