发明名称 |
Recombinant proteolytic tryptases, active site mutants thereof, and methods of making same |
摘要 |
Disclosed is a method of expressing enzymatically-active, recombinant proteolytic tryptase in a eukaryotic host cell, expression constructs which drive the production of enzymatically-active tryptase in transformed hosts, and genetically-engineered eukaryotic host cells containing the expression constructs and which express enzymatically-active proteolytic tryptases. Uses for the proteolytic tryptases so produced are also disclosed. Also disclosed is a method of making active site mutants of proteolytic tryptases in a eukaryotic host cell, expression constructs which drive the production of the mutants in transformed eukaryotic host cells, and genetically-engineered eukaryotic host cells containing the expression constructs and which express the active-site mutated form of proteolytic tryptases.
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申请公布号 |
US6998260(B1) |
申请公布日期 |
2006.02.14 |
申请号 |
US20000598982 |
申请日期 |
2000.06.21 |
申请人 |
PROMEGA CORPORATION |
发明人 |
MAFFITT MARK;NILES ANDREW L.;HAAK-FRENDSCHO MARY |
分类号 |
C12N9/64;C12N15/09;C07K16/40;C12N1/14;C12N1/19;C12N5/00;C12N9/50;C12N15/00;C12N15/81;C12P21/06;C12P21/08;C12Q1/37;C12R1/84 |
主分类号 |
C12N9/64 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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