| 摘要 |
FIELD: biology, toxicological and sanitary chemistry, in particular method for determination of O-(2,3-dihydro-2,2-dimethyl-7-benzofuranyl)-N-methylcarbamate in biological sample, useful in chemical, toxicological and veterinary laboratories, etc. ^ SUBSTANCE: claimed method includes grinding of biological tissue, double treatment thereof with ethyl acetate and acetone mixture in volume ratio of 1:1, for 30 min in each case, extractant evaporation, and residue dissolution in diethyl ether, introducing of obtained solution into silica gel column, solvent evaporation and chromatography. For chromatography hexane/acetone in ratio of 9:1 is used as mobile phase. Then effluent fractions containing O-(2,3-dihydro-2,2-dimethyl-7-benzofuranyl)-N-methylcarbamate are conjugated, eluent is evaporated, and residue is dissolved in acetonitrile/water (5:5) solvent mixture and subjected to chromatography by HPLC using acetonitrile/water (5:5) as mobile phase and sensor based on photodiode matrix. ^ EFFECT: method of increased sensitivity and accuracy. ^ 3 tbl, 2 ex |
