| 摘要 |
FIELD: medicine, preparative biochemistry. ^ SUBSTANCE: invention relates to a method for preparing, isolating, purifying and stabilizing of the human granulocytic colony-stimulating factor (HCSF) that can be used for medicinal aims. Method involves disrupting recombinant bacterial cells, isolation of bodies inclusion, removal of inert bacterial proteins, recovery, renaturation, purifying by chromatography method and stabilization of HCSF. Also, invention relates to the immunobiological agent based on thereof. Invention provides high yield, quality and stability of HCSF. ^ EFFECT: improved preparing method. ^ 4 cl, 5 dwg, 6 tbl, 16 ex |
