Method and apparatus for analysis and sorting of polynucleotides based on size

摘要

The invention relates to a microfabricated device and methods of using the device for analyzing and sorting polynucleotide molecules by size.

主权项

1. A method of analyzing an individual polynucleotide single-stranded DNA molecule in a solution using a microfluidic device, the method comprising :
transporting the individual molecule through a detection region having a volume of between 1 femtoliter and 1 picoliter that is positioned in a microfluidic channel of the device without hydrodynamically focusing the solution with a sheath fluid, and without previous size separation of polynucleotides in the solution; and determining the size of the molecule as it passes through the detection region.