Nukleotidsequenzen, welche für das dapF-Gen kodieren

摘要

The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which is identical to the extent of at least 70 % to a polynucleotide which codes for the polypeptide which is expressed by the dapF gene contained on vector pEC-XT99A-dapF in the deposited C.glutamicum strain DSM 12969, c) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70 % to the amino acid sequence of SEQ ID No. 2, d) polynucleotide which is complementary to the polynucleotides of a), b) or c), and e) polynucleotide comprising at least 15 successive bases of the polynucleotide sequence of a), b), c) or d), and a process for the fermentative preparation of L-Lysin with amplification of the dapF gene.