发明名称 |
GENOMIC AND PROTEOMIC APPROACHES FOR THE DEVELOPMENT OF CELL CULTURE MEDIUM |
摘要 |
<p>The present invention relates to a method for designing a cell culture medium adapted to support a cell line in a pre-defined manner. The method includes generating an expression profile of a cell line, identifying from the expression profile a set of biomolecules to evaluate for their effect on an end-point assay using the cell line, testing each biomolecule in the set for its effect in the endpoint assay, wherein each biomolecule that is determined to have a measurable effect in the end-point assay relative to a control, not containing the biomolecule, is considered a positive biomolecule and formulating a cell culture medium for the cell line by adding a positive biomolecule to a basal medium to form a modified medium, and determining whether the modified medium is sufficient to support the cell line in the pre-defined manner. Methods for identifying biomolecules for use in designing a cell culture medium adapted to support a cell line in a pre-defined manner, methods for preparing a serum-free cell culture medium that is sufficient to support a cell line and arrays for use in such methods are also provided.</p> |
申请公布号 |
EP1623020(A2) |
申请公布日期 |
2006.02.08 |
申请号 |
EP20040760957 |
申请日期 |
2004.05.10 |
申请人 |
SIGMA-ALDRICH CO. |
发明人 |
DONAHUE, LAUREL M.;ALLISON, DANIEL W.;JOHNSON, TERRELL K.;ABOYTES, KATHRYN A.;FONG, DANNY |
分类号 |
C12N5/02;C12N5/00;C12N5/06;C12Q;C12Q1/68;(IPC1-7):C12N5/02 |
主分类号 |
C12N5/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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