<p>A method for balancing multiplexed PCR methods is provided. In the method, two or more sequential temporal PCR stages are used to effectively separate two or more PCR reactions in a single tube as an alternative to primer limiting to modulate the relative rate of production of a first amplicon by a first primer set and a second amplicon by a second primer set during the first and second amplification stages. Also provided are rapid RT-PCR methods that find particular use in intraoperative diagnoses and prognoses, for instance in diagnosing malignant esophageal adenocarcenoma by determining expression levels of carcinoembryonic antigen (CEA) in sentinel lymph nodes.</p>
申请公布号
EP1373569(A4)
申请公布日期
2006.02.08
申请号
EP20020715027
申请日期
2002.03.04
申请人
UNIVERSITY OF PITTSBURGH OF THE COMMONWEALTH SYSEM OF HIGHER EDUCATION
发明人
GODFREY, TONY, E.;LUKETICH, JAMES, D.;RAJA, SIVA;KELLY, LORI, A.;FINKELSTEIN, SYDNEY, D.