发明名称 |
Thermophilic amino acid biosynthesis system enzyme gene of thermotolerant coryneform bacterium |
摘要 |
A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from Corynebacterium thermoaminogenes, preferably an enzyme that functions at a higher temperature compared with that of Corynebacterium glutamicum. PCR is performed by using the primers and chromosomal DNA of Corynebacterium thermoaminogenes as a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of Corynebacterium thermoaminogenes.
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申请公布号 |
US6995250(B1) |
申请公布日期 |
2006.02.07 |
申请号 |
US20020089057 |
申请日期 |
2002.04.03 |
申请人 |
AJINOMOTO CO., INC. |
发明人 |
HIRANO SEIKO;KIMURA EIICHIRO;OSUMI TSUYOSHI;MATSUI KAZUHIKO;KAWAHARA YOSHIO;NONAKA GEN;MATSUZAKI YUMI;AKIYOSHI NAOKI;NAKAMURA KANAE;KURAHASHI OSAMU;NAKAMATSU TSUYOSHI;SUGIMOTO SHINICHI |
分类号 |
C07H21/02;C07H21/04;C07K14/34;C12N9/02;C12N9/04;C12N9/12;C12N9/26;C12N9/88;C12N15/31;C12N15/53;C12N15/54;C12N15/56;C12N15/60;C12P21/06 |
主分类号 |
C07H21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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