发明名称 |
Methods and compositions for the preparation and use of fixed-treated cell-lines and tissue in fluorescence in situ hybridization |
摘要 |
This invention relates to methods for the detection of one or more mRNA transcripts in paraffin-embedded tissue by "mRNA liberation in fixed-treated tissue or 'MLIFTT'". This method includes treating the tissue with ammonia-ethanol and sodium borohydride combined with pressure cooking of the tissue. The chemical treatments reduce the tissue autofluorescence and the physical treatments overcome the interference created by the fixation-induced chemical bonds. The methods of the present invention can be utilized to identify a plurality of mRNA transcripts in a microarray format.
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申请公布号 |
US6995020(B2) |
申请公布日期 |
2006.02.07 |
申请号 |
US20030624233 |
申请日期 |
2003.07.21 |
申请人 |
AUREON LABORATORIES, INC. |
发明人 |
CAPODIECI PAOLA;EDELSON JON |
分类号 |
G01N33/00;C07H21/00;C07H21/04;C12M1/34;C12Q1/68 |
主分类号 |
G01N33/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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