VERFAHREN ZUR VERBESSERTEN HERSTELLUNG UND ISOLIERUNG VON TRANS-DIHYDROXY-CYCLOHEXADIEN-CARBONSÄUREN UND/ODER DEREN FOLGEPRODUKTE SOWIE EIN DAZU GEEIGNETER GENETISCH VERÄNDERTER ORGANISMUS

摘要

In the production of genetically-modified, non-pathogenic organisms particularly an improved production and isolation of trans-dihydroxycyclohexadiene carboxylic acids and/or derivatives thereof, the synthesis of the transhydroxy cyclohexadiene carboxylic acids is performed in an enantiomer-pure form without detectable impurities with chorismate by cultivation of at least one genetically altered non-pathogenic organism which has an aromate embolism and has, in comparison with a corresponding, genetically not altered organism, an increased activity of the isochorismate synthase (EntC) and isochorismatase (EntB) and an activity of the 2,3 dihydroxybenzoate synthase (EntA) which is close to zero, or an increased activity of the isochorismatase (EntB) and a reduced activity of the isochorismate synthase (EntC) and an activity of the 2,3-dihydroxybenzoat-synthase (entA) of close to zero.