METHOD OF PURIFYING VIRUS ENVELOPE

摘要

It is intended to provide a method of industrially purifying the envelope of a virus (for example, Sendai virus (Hemagglutinating Virus of Japan, HVJ)). More specifically, it is intended to provide a method whereby an inactivated virus envelope is purified by the combined use of ion exchange chromatography with hydrophobic chromatography to thereby purify the envelope at a high yield while sustaining the cell fusion activity of the virus. The virus envelope thus purified is usable as a vector for transferring a biopolymer such as a gene into cells or living body. This method is also applicable to the purification of an attenuated envelope virus.