METHOD OF PRODUCING TARGET PROTEIN, FUSED PROTEIN AND GENE THEREOF, PARTIAL SEQUENCE PROTEIN OF INTEIN AND GENE THEREOF, EXPRESSION VECTOR AND TRANSFORMANT

摘要

It is intended to provide techniques whereby a target protein is efficiently obtained as a normal protein even in the case of a protein which can be hardly expressed by usual recombinant DNA techniques. A fusion protein composed of a molecular chaperone, an intervening protein having an activity of cleaving a peptide bond, and a target protein is prepared, from which fusion protein the target protein is cleaved by the action of the intervening protein having the activity of cleaving a peptide bond. Examples of the intervening protein having the activity of cleaving a peptide bond include an intein and a protein consisting of a partial sequence of the intein.