摘要 |
Described is an oligonucleotide for analyzing a target nucleotide sequence by hybridisation and applications thereof. The oligonucleotide has the following general structure. 5'-Xp-Yq-Zr-3' or 5'-Zr-Yq-Xp-3' wherein Xp represents a first hybridisation portion having a specific hybridising nucleotide sequence substantially complementary to said target nucleotide sequence in said sample nucleic acid to hybridise therewith; Yq represents a regulator portion comprising at least two universal bases or non-discriminatory base analogues; Zr represents a second hybridisation portion having a pre-selected arbitrary nucleotide sequence; p, q and r represent the number of nucleotides; and X,Y and Z is deoxyribonucleotide or ribonucleotide.
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