| 摘要 |
Disclosed is a recombinant mutant nitroreductase encoded by a mutated E. coli NFSB gene, wherein the nitroreductase comprises a substitution of at least one of serine 40, threonine 41, tyrosine 68, phenylalanine 70, aspragine 71, glycine 120 or phenylalanine 124 and has an increased nitroreductase activity for CB1954 compared to the wild-type enzyme. The nitroreductase or an isolated polynucleotide encoding it, is used in the manufacture of a medicament for the treatment of cancer by conversion of a prodrug into an active cytotoxic compound.
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