NOVEL SENSORS WITH NANOPARTICLE PROBE

摘要

The present invention concerns a method for the detection of the presence of an analyte in a sample, the analyte being one member of an enzyme/substrate pair, the method comprising: (a) providing nanoparticles of a transition metal, the other member of said pair; an electron acceptor and a transition metal salt capable to undergo a redox reaction with an electron donor thereby depositing a metal on said nanoparticles; (b) providing conditions enabling enzymatically catalyzed reaction between the substrate and the electron acceptor; (c) detecting a change in size of the nanoparticle; an enlargement of the nanoparticles' size indicating the presence of said one member in the sample.