| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for clearly and simply detecting a nucleic acid sequence in a specimen, capable of obtaining rapidly and easily a result in a good reproducibility, since without requiring complex operations. SOLUTION: This method for detecting the nucleic acid sequence contained in the specimen solution by performing an amplification reaction by using an oligonucleotide for the nucleic acid sequence amplification in a specimen solution containing the specific nucleic acid sequence, at the same time with or after the amplification reaction, bringing the above in contact with an oligonucleotide bonded with a ligand capable of specifically bonding with the nucleic acid sequence and detecting the ligand bonded with the specific nucleic acid sequence is characterized by using a liquid-passing filter bonded with the oligonucleotide specifically recognizing the amplified nucleic acid sequence, and a labeled physiologically active substance. COPYRIGHT: (C)2006,JPO&NCIPI
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