| 摘要 |
PROBLEM TO BE SOLVED: To provide a confocal scanning type microscope capable of intuitively setting three-dimensional scanning areas for a plurality of observation objects scattered in the space of a sample and performing detailed scanning to the respective scanning areas, and to provide a three-dimensional scanning area designating method. SOLUTION: The confocal scanning type microscope is equipped with an objective to condense emitted light from a light source on the sample, a function to scan along a sample surface with converged light, and a photodetector to detect the intensity of light transmitted through a pinhole arranged at a position conjugate to the light focusing position of the objective by relatively moving the light focusing position of the objective and the position of the sample in the optical axis direction of the converged light. In the microscope, a three-dimensional image is constituted of a plurality of two-dimensional images acquired while moving a moving mechanism and the constituted three-dimensional image is displayed. Then, the scanning area is set based on information obtained by area designation to designate a noticeable area on the displayed three-dimensional image. COPYRIGHT: (C)2006,JPO&NCIPI
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