| 摘要 |
<p>It is intended to provide a method of detecting a virus which comprises detecting, from a detection subject, a nucleic acid of a base sequence consisting of 10 or more consecutive bases selected from a sequence corresponding to the base sequence represented by SEQ ID NO:1 that is the overlap of the ORF2 region and the ORF3 region in a hepatitis E virus gene, and regarding the subject as positive to the hepatitis E virus in the case where the above nucleic acid is detected; and a detection kit for the embodiment of the method. According to this method, a comprehensive or selective system of detecting HEV at a high sensitivity can be established. This detection method is applicable to blood for transfusion, blood preparations, foods, environmental examinations and so on. Moreover, a genotype-specific detection system is usable in clarifying infection route and epidemiological studies.</p> |
