Method to identify and analyze genes having modified expression in stimulated T cells

摘要

A method of identifying genes involved in the stimulation of primary T cells comprising the steps of: a) contacting experimental cells with a stimulating agent; b) preparing RNA from said experimental cells at one or more stimulation phases; c) measuring the level of gene expression in the cells; d) comparing the levels of gene expression of said experimental cells to the level of gene expression in control cells that have not been exposed to an stimulation agent; e) identifying genes that are up regulated or down regulated in said experimental cells relative to said control cells.