CLEAVAGE OF FUSION PROTEINS USING GRANZYME B PROTEASE

摘要

A method for the preparation of a polypeptide of interest in authentic form by enzymatic cleavage of fusion proteins using Granzyme B protease (EC 3.4.21.79). There is also provided fusion proteins comprising a polypeptide of interest an a fusion partner, wherein the junction region between the polypeptide of interest and the fusion partner comprises a Grazyme B protease cleavage site adjacent to the polypeptide of interest, and a human Granzyme B protease variant wherein the Cystein residue no. 228 (chymotrupsinogen numbering) is mutated to Phenylalanine.