| 摘要 |
FIELD: biotechnology, microbiology, medicine, pharmacy. ^ SUBSTANCE: invention relates to a method for preparing and concentrating cytotoxic substances, in particular, epotilones and their using for preparing pharmaceutical compositions used in treatment of proliferative diseases. Method for concentrating epotilones involves addition of a complex-forming component taken among the group of cyclodextrins to an aqueous cultural medium used in growing the producer, - Sorangium cellulosum. Also, invention describes a cultural medium used for growing S. cellulosum that contains the abovementioned complex-forming component and a method for preparing epotilones by using this medium. Invention represents a method for separating prepared epotilones being primarily epotilones A and B by method of reverse column chromatography and a (lower)-alkylcyanide containing eluent. Invention describes crystalline form of epotilone B and its using in pharmaceutical composition for treatment of proliferative diseases and a method for treatment of these diseases. Invention provides preparing epotilones in the industrial scale. ^ EFFECT: improved preparing method. ^ 16 cl, 6 tbl, 4 ex |
