DETECTION AND ASSAY OF CYTOMEGALOVIRUS

摘要

PROBLEM TO BE SOLVED: To provide detection and assay of CMV that can rapidly amplify and detect the β2.7 gene originating from CMV at a certain temperature through a one-step operation.

SOLUTION: The mRNA of β2.7 gene originating from CMV is amplified by utilizing the combination of specifically amplifiable oligonucleotide primers and the RNA amplification step comprising these combinations of oligonucleotide primers is measured and detected by using an oligonucleotide probe that is marked with an intercalated fluorescent dye.

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