| 摘要 |
The present invention relates to apparatus, systems, and methods for analyzing biological samples. In particular, the invention provides systems and methods for analyzing samples in a light microscope. The invention allows microscopic position sensing and focusing. The invention includes the use of at least two coordinated beams of light, one of which operates to determine the position of the other. In a preferred embodiment, the system is a microscope having total internal reflection optics installed. Systems of the invention can also be constructed from a standard microscope configured with a total internal reflection objective.
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