发明名称 |
MAGNETIC ENZYME DETECTION TECHNIQUES |
摘要 |
<p>A diagnostic test kit for detecting the presence or quantity of an enzyme or enzyme inhibitor is provided. The diagnostic kit utilizes reactive complexes to facilitate the detection of the enzyme or enzyme inhibitor. The reactive complexes include a substrate joined (e.g., covalently bonded, physically adsorbed, etc.) to a reporter and magnetic substance. In one embodiment, for example, a peptide, protein, or glycoprotein substrate is joined to a reporter (e.g., dyed latex particle) and magnetic particle. In this embodiment, the substrate provides a cleavage target for a proteolytic enzyme. Specifically, upon contacting the reactive complexes, the proteolytic enzyme cleaves the substrate and releases the reporter and/or magnetic particle. The signal exhibited by the released reporters may then be used to indicate the presence or quantity of an enzyme or enzyme inhibitor within the test sample.</p> |
申请公布号 |
WO2006007009(A1) |
申请公布日期 |
2006.01.19 |
申请号 |
WO2005US14169 |
申请日期 |
2005.04.25 |
申请人 |
KIMBERLY-CLARK WORLDWIDE, INC.;SONG, XUEDONG;YANG, SHU-PING;KAYLOR, ROSANN, MARIE, MATTHEWS |
发明人 |
SONG, XUEDONG;YANG, SHU-PING;KAYLOR, ROSANN, MARIE, MATTHEWS |
分类号 |
(IPC1-7):G01N33/543;G01N33/58;G01N33/68;C12Q1/04 |
主分类号 |
(IPC1-7):G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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