METHOD FOR PREPARING HIGH-PURITY AGAROSE BY USING RECOMBINANT ARYLSULFATASE

摘要

Disclosed herein is a method for preparing high-purity agarose by using arylsufatase. The method comprises ligating arylsulfatase gene derived from Pseudoalteromonas carrageenovora into an expression vector, inserting the expression vector into a microorganism for transformation, culturing the transformed microorganism for mass production of the recombinant arylsulfatase and reacting the recombinant arylsufatase with agar to produce high-purity agarose. According to the present invention, it is possible to realize localization of agarose for biotechnological and medical use which as depended on importation so far and to avoid excessive use of organic solvents which is a main problem in the conventional production process of agarose by substituting an enzymatic production process. Thus, the amount of the used organic solvents can be reduced and waste treatment plant for the solvent Recovery process may be minimized, providing positive economic effect Such as import substitution effect and foreign export opportunity.