Imaging method with light scanning microscope having a point source light distribution

摘要

The light scanning microscope as several lasers of different colors whose beams are combined to make a scanning beam. The beam may be scanned over a microscope slide with stationary and moving objects. The various scans include a fine reference scan and four successive coarse scans. The scan lines of each scan lie in the spaces between the lines of the previous scan. A pictorial representation of the object under investigation has scan mechanism capable of coarse and fine scans which may be performed in turn to give high time and spatial resolution.