| 摘要 |
PROBLEM TO BE SOLVED: To provide a method for identifying an agent for controlling PDE10A activity, and to provide rat PDE10A polynucleotide and polypeptide sequences. SOLUTION: This method of screening for an agent that inhibits intracellular phosphodiesterase 10A activity, comprising administering an agent to striatal medium spiny neurons and submaximally activating adenylate cyclase, administering an agent to striatal medium spiny neurons and submaximally activating guanylate cyclase, measuring cAMP generation and cGMP generation in the cells, and calculating the cAMP EC<SB>200</SB>and the cGMP EC<SB>200</SB>, wherein the agent is identified as a PDE10A inhibitor if the ratio of cAMP EC<SB>200</SB>/cGMP EC<SB>200</SB>is comparable to the ratio produced by administration of papaverine under the same assay conditions. Also, rat PDE10A polynucleotide and polypeptide sequences are obtained. COPYRIGHT: (C)2006,JPO&NCIPI
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