VARIANT XYLITOL DEHYDROGENASE ENZYME, MICROORGANISM PRODUCING THE SAME AND METHOD FOR CONVERTING XYLITOL TO XYLULOSE USING THE ENZYME OR MICROORGANISM

摘要

PROBLEM TO BE SOLVED: To provide a variant xylytol dehydrogenase (XDH) having improved conversion efficiency of xylitol to xylulose by improving the coenzyme demand of wild XDH to nicotinamide adenine dinucleotide phosphate (NADP) type and improving the heat-resistance.

SOLUTION: The coenzyme demand of a wild XDH is changed to NADP demand by substituting the 207th aspartic acid of the amino acid sequence to alanine, 208th isoleucine to arginine, 209th phenylalanine to threonine or tyrosine and 211th asparagine to arginine and the heat-resistance is improved by substituting the 96th serine to cysteine, the 99th serine to cysteine and 102nd tyrosine to cysteine and introducing a structure-stabilized zinc bonding site.

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