VIRAL PARTICLE-LIKE CONSTRUCT AND METHOD OF FORMING THE SAME UNDER PHYSIOLOGICAL CONDITIONS

摘要

It is intended to provide a novel method of forming uniform viral particles under physiological conditions. Namely, a method of forming a mass of viral particles in a uniform size made of a viral protein, characterized by comprising incubating a viral protein (for example, VP1 of SV40 virus) at pH 5.0 to pH 7.0 and at room temperature in the presence of 130 mM to 170 mM of sodium chloride and 1.5 mM to 2.5 mM of a divalent cation and in the presence of a particle-formation promoter such as VP2 of SV40. In the case where it is needed to enclose a substance in the viral particles, the substance to be enclosed may be allowed to coexist in the incubation as described above.