TEMPERATURE- AND PHOTO-RESPONDING COMPOSITION AND CELL-CULTURING SUBSTRATE PRODUCED FROM THE SAME

摘要

PROBLEM TO BE SOLVED: To provide a cell-culturing substrate having a temperature- and photo-responding surface whose cell adhesiveness can be locally raised or reduced with a high contrast and reversibility at a specified region by photo-irradiation before and/or after seeding a cell, and to provide a temperature- and photo-responding composition comprised of the same.

SOLUTION: The temperature- and photo-responding composition comprises an acrylamide-based monomer component, that composes a temperature-responding polymer, and a monomer component having a spirobenzopyran and/or spirooxazine residue having photoresponsiveness and contains at least as one component a photo-responding copolymer whose cell adhesiveness changes responding to photoirradiation and/or to temperature change, thus the composition provides a measure for fractional recovery of only a desired cell or a cell group selectively without injuring activity of a cultured cell or without any complicated operation for incubation according to a desired pattern.

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