Carbon electrode surface for attachment of DNA and protein molecules

摘要

Methods for conducting an assay to detect nucleic acid hybridization are disclosed. In particular, the fabrication of a carbon electrode suited to nucleic acid hybridization detection is described. In some preferred embodiments, a micro array of carbon electrodes is constructed using photolithography. The final step in the photolithography process involves developing a solder mask with an alkaline solution. The alkaline solution oxidizes the carbon surface producing surface carboxylic acid groups. The surface carboxylic acid groups are reacted with EDC or DCC and NHS to produce NHS esters. Immobilization of NeutrAvidin(TM) onto the electrode surface is effected by reaction with the surface NHS ester groups. A biotinylated probe DNA molecule is then attached to the electrode via binding between the biotin group and the immobilized NeutrAvidin(TM). The resulting electrode can be used to detect hybridization between the probe DNA and complementary target DNA.